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Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantitative Polymerase Chain Reaction-baserede analyser af Murine tarmens mikrobiota efter peroral antibiotisk behandling
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Kvantitative Polymerase Chain Reaction-baserede analyser af Murine tarmens mikrobiota efter peroral antibiotisk behandling

Article DOI: 10.3791/58481-v • 08:33 min • November 17th, 2018
November 17th, 2018 •
1,2, 1,2, 1,2
1The Peter Gorer Department of Immunobiology, King's College London, 2The Francis Crick Institute

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Her leverer vi detaljerede protokoller til oral administration af antibiotika til mus, samling af fækal prøver, DNA-ekstraktion og kvantificering af fækal bakterier af qPCR.

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Immunologi og infektion sag 141 tarmens mikrobiota antibiotika 16S rRNA fækal bakterier qPCR fækal prøvetagning
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