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Biochemistry

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JoVE Journal Biochemistry
Udtryk og rensning af nuklease-fri oxygen Scavenger Protocatechuate 3, 4-Dioxygenase
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Udtryk og rensning af nuklease-fri oxygen Scavenger Protocatechuate 3, 4-Dioxygenase

Article DOI: 10.3791/59599-v • 10:14 min • November 8th, 2019
November 8th, 2019 •
1, 1, 1, 1
1Cancer Biology and Genetics, College of Medicine, The Ohio State University

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Protocatechuate 3, 4-dioxygenase (PCD) kan enzymatisk fjerne fri diatomisk ilt fra et vandigt system ved hjælp af dets substrat protocatechuinsyre Acid (PCA). Denne protokol beskriver udtrykket, rensning og aktivitetsanalyse af dette iltskylle enzym.

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Biokemi kromatografi protocatechuate-3 4-dioxygenase (PCD) nukleasekontaminering protocatechuinsyre Acid (PCA) reaktive ilt arter ilt scavenging Systems (OSS)
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