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Journal / Biochemistry / 生物层干涉测量(BLI)在转录中研究蛋白质-蛋白质相互作用的应用…
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Biochemistry

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JoVE Journal Biochemistry
生物层干涉测量(BLI)在转录中研究蛋白质-蛋白质相互作用的应用
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生物层干涉测量(BLI)在转录中研究蛋白质-蛋白质相互作用的应用

Article DOI: 10.3791/59687-v • 07:18 min • July 26th, 2019
July 26th, 2019 •
1,2, 3, 1,2
1Department of Pharmacology, Robert Wood Johnson Medical School, Rutgers University, 2Graduate Program in Physiology and Integrative Biology, School of Graduate Studies, Rutgers University, 3Department of Plant Biology, School of Environmental and Biological, Rutgers University

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转录因子(TFs)与RNA聚合酶的相互作用通常使用下拉测定进行研究。我们应用生物层干涉测量(BLI)技术来描述GrgA与衣原RNA聚合酶的相互作用。与下拉检测相比,BLI可检测实时关联和解结,具有更高的灵敏度,并且具有高度定量性。

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本月在JoVE 第149期 生物层干涉测量 衣原体 CT504 TC0791 GrgA 蛋白质-蛋白质相互作用 转录因子 转录调节
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