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Immunology and Infection

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Intravital Imaging av intraepitelial lymfocyter i murina tunn tarmen
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Intravital Imaging av intraepitelial lymfocyter i murina tunn tarmen

Article DOI: 10.3791/59853-v • 08:00 min • June 24th, 2019
June 24th, 2019 •
1, 1
1Center for Immunity and Inflammation, Department of Pathology, Immunology and Laboratory Medicine, Rutgers New Jersey Medical School

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Vi beskriver en metod för att visualisera GFP-märkta γδ IELs med intravital avbildning av murina tunn tarmen genom inverterad spinning disk konfokalmikroskopi. Denna teknik gör det möjligt att spåra levande celler i slem hinnan i upp till 4 h och kan användas för att undersöka en mängd olika intestinal immun-epitelial interaktioner.

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Immunologi och infektion immunologi T-cell intraepitelial lymfocyter mus tunn tarmen intravital Imaging spinning disk konfokalmikroskopi cell spårning
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