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Tumorsphere afleiding en behandeling van primaire tumor cellen geïsoleerd van de muis Rhabdomyosarcomas

Article DOI: 10.3791/59897-v • 09:21 min • September 13th, 2019
September 13th, 2019 •
1,2,3, 2
1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

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Dit protocol beschrijft een reproduceerbare methode voor isolatie van muizen rhabdomyosarcoom primaire cellen, tumorsphere vorming en behandeling, en transplantaat transplantatie vanaf tumorspheres culturen.

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Genetica probleem 151 tumorspheres rabdomyosarcoom skeletspieren primaire cellen recombinant eiwit behandeling plasmide transfectie
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