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Journal / Biochemistry / In Vivo Hydroxyl Radical Protein Footprinting…
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Biochemistry

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JoVE Journal Biochemistry
In Vivo Hydroxyl Radical Protein Footprinting para o Estudo de Interações Proteicas em Caenorhabditis elegans
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In Vivo Hydroxyl Radical Protein Footprinting para o Estudo de Interações Proteicas em Caenorhabditis elegans

Article DOI: 10.3791/60910-v • 08:00 min • April 1st, 2020
April 1st, 2020 •
1, 1
1Department of Pharmaceutical Sciences, University of Maryland

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In vivo a oxidação fotoquímica rápida de proteínas (IV-FPOP) é uma técnica de pegada de proteína radical hidroxil que permite o mapeamento da estrutura proteica em seu ambiente nativo. Este protocolo descreve a montagem e a configuração do sistema de fluxo microfluido IV-FPOP.

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Bioquímica Edição 158 Caenorhabditis elegans,pegada de proteína radical hidroxila FPOP espectrometria de massa in vivo proteômica
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