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Flow Cytometry Analyse av immuncelle undergrupper innenfor Murine Milten, Benmarg, Lymfeknuter og synovialvev i en slitasjegikt modell
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Flow Cytometry Analyse av immuncelle undergrupper innenfor Murine Milten, Benmarg, Lymfeknuter og synovialvev i en slitasjegikt modell

Article DOI: 10.3791/61008-v • 12:23 min • April 24th, 2020
April 24th, 2020 •
1,2, 1,3, 1, 1, 1, 1
1Raymond Purves Bone and Joint Research Laboratory, Institute of Bone and Joint Research, Kolling Institute, Royal North Shore Hospital, University of Sydney, 2HTRG - Heidelberg Trauma Research Group, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Trauma and Reconstructive Surgery, Heidelberg University Hospital, 3Department of Large Animal Clinical Sciences, College of Veterinary Medicine, Michigan State University

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Her beskriver vi en detaljert og reproduserbar strømningscytometriprotokoll for å identifisere monocytt/makrofag og T-celleundergrupper ved hjelp av både ekstra- og intracellulære fargingsanalyser innenfor murinsplitten, benmargen, lymfeknuter og synovialvev, ved hjelp av en etablert kirurgisk modell av murinslitasjegikt.

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Medisin Utgave 158 Slitasjegikt immunologi strømningscytometri lymfocytter undergrupper T-celler monocytter makrofager ortopedi posttraumatisk slitasjegikt
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