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JoVE Journal Developmental Biology
Differentiering og karakterisering af neurale forfædre og neuroner fra museembryst stamceller
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Differentiering og karakterisering af neurale forfædre og neuroner fra museembryst stamceller

Article DOI: 10.3791/61446-v • 08:47 min • May 15th, 2020
May 15th, 2020 •
1, 1, 1, 1
1Departments of Biochemistry and Molecular Biology, Penn State College of Medicine, Penn State Hershey Cancer Institute

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Vi beskriver proceduren for in vitro differentiering af mus embryonale stamceller i neuronale celler ved hjælp af hængende dråbe metode. Derudover udfører vi en omfattende fænotypisk analyse gennem RT-qPCR, immunofluorescence, RNA-seq og flowcytometri.

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Udviklingsbiologi Udgave 159 Mus embryonale stamceller embryonale organer neurale stamfader celle neuroner differentiering hængende dråbe E14
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