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Isolering av proksimale væsker for å undersøke tumormikromiljøet i bukspyttkjerteladenokarsinom

Article DOI: 10.3791/61687-v • 05:44 min • November 5th, 2020
November 5th, 2020 •
*1, *2, 1,3, 1,3, 4,5,6, 1,3, 2,7, 2
1Section of Pancreatic Surgery, Humanitas Clinical and Research Center-IRCCS, 2Department of Immunology and Inflammation, Humanitas Clinical and Research Center-IRCCS, 3Department of Biomedical Sciences, Humanitas University, 4Giotto Biotech S.R.L., 5Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, 6National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, 7Department of Medical Biotechnology and Translational Medicine, University of Milan
* These authors contributed equally

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Bukspyttkjerteljuice er en verdifull kilde til biomarkører for kreft i bukspyttkjertelen. Vi beskriver her en metode for intraoperativ innsamlingsprosedyre. For å overvinne utfordringen med å vedta denne prosedyren i murine modeller, foreslår vi en alternativ prøve, tumor interstitiell væske, og beskriver her to protokoller for isolasjon.

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Kreftforskning utgave 165 pankreas adenokarsinom bukspyttkjerteljuice tumor interstitiell væske proksimal væske flytende biopsi biomarkører proteomikk
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