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Journal / Genetics / 使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变…
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Genetics

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JoVE Journal Genetics
使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变
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使用下一代测序来鉴定与CD4启动子附近CAS9诱导的双链断裂修复相关的突变

Article DOI: 10.3791/62583-v • 06:59 min • March 31st, 2022
March 31st, 2022 •
*1, *2, 1, 1, 2,3
1Division of Biology, Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory, Kansas State University, 3Department of Diagnostic Medicine/Pathobiology, Kansas State University
* These authors contributed equally

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这里介绍的是sgRNA / CAS9内切酶和下一代测序方案，可用于鉴定与CD4启动子附近的双链断裂修复相关的突变。

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遗传学，第181期，DNA损伤反应，DSB，DSB修复，CAS9诱导的DSB，下一代测序，基因组学
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