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In vitro Karakterisering af histon chaperoner ved hjælp af analytiske, pull-down og chaperoning assays

Article DOI: 10.3791/63218-v • 08:16 min • December 29th, 2021
December 29th, 2021 •
*1,2, *1,2, 1,3, 1,2, 1,2, 1, 1,4, 1,5, 1, 1
1Institute of Life Sciences, 2Regional Centre for Biotechnology, 3School of Biotechnology, KIIT University, 4Department of Molecular Biophysics and Biochemistry, Yale University, 5Department of Pharmacology, University of Vermont College of Medicine
* These authors contributed equally

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Denne protokol beskriver et batteri af metoder, der inkluderer analytisk størrelsesekskluderingskromatografi til at studere histon chaperone oligomerisering og stabilitet, pull-down assay for at opklare histon chaperon-histon-interaktioner, AUC til at analysere støkiometrien af proteinkomplekserne og histon chaperoning assay for funktionelt at karakterisere en formodet histon chaperone in vitro.

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Biokemi udgave 178 histon chaperon nukleosomsamling kromatin nukleoplasmin NAP plasmid supercoiling analytisk ultracentrifugering
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