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Flowcytometrisk analyse av biomarkører for påvisning av humane spermfunksjonsdefekter
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Flowcytometrisk analyse av biomarkører for påvisning av humane spermfunksjonsdefekter

Article DOI: 10.3791/63790-v • 08:48 min • April 21st, 2022
April 21st, 2022 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1
1Institute of Toxicology, College of Preventive Medicine, Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine, Army Medical University (Third Military Medical University)

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Den nåværende protokollen gir en praktisk løsning som muliggjør måling av apoptose, mitokondriemembranpotensial og DNA-skade i menneskelig sæd med et enkelt cytometer.

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Flowcytometri biomarkører spermfunksjonelle defekter konvensjonelle sædparametere idiopatisk infertilitet spermapoptose mitokondriemembranpotensial (MMP) DNA-skade FCM-teknikker vedlegg V-fluoresceinisotiocyanat (FITC)/propidiumjodid (PI) farging 5,5& 8242;,6,6& 8242;-tetraklor-1,1& 8242;,3,3& 8242;-tetraetyl-benzimidazolylkarbocyaninjodid (JC-1) sonde spermkromatinstrukturanalyse (SCSA) akridin oransje (AO) farging
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