Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Medicine / Rectal Organoid Morphology Analysis (ROMA): A…
JoVE Journal
Medicine

Se requiere una suscripción a JoVE para ver este contenido.

JoVE Journal Medicine
Rectal Organoid Morphology Analysis (ROMA)
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
Journal (Medicine)
TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents
Remote Laboratory Management: Respiratory Virus Diagnostics
Journal (Medicine)
Remote Laboratory Management: Respiratory Virus Diagnostics
 

Rectal Organoid Morphology Analysis (ROMA): A Diagnostic Assay in Cystic Fibrosis

Article DOI: 10.3791/63818-v • 07:56 min • June 10th, 2022
June 10th, 2022 •
1,2, 1, 3,4, 5, 6, 7,8, 7,9, 8, 3,4, 1,2, 1,2, 10,11, 1,2, 1,2
1Department of Development and Regeneration, Woman and Child Unit, CF research lab, KU Leuven, 2Department of Pediatrics, Pediatric Pulmonology, University Hospitals Leuven, 3VIB Bio Imaging Core, VIB-KU Leuven Center for Brain & Disease Research, 4Department for Neuroscience, KU Leuven, 5Interuniversity Center for Biostatistics and Statistical Bioinformatics, University of Leuven and University of Hasselt, 6Department of Pediatrics, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 7Department of Chronic Diseases and Metabolism (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, 8Department of Development and Regeneration, Stem Cell Institute Leuven (SCIL), KU Leuven, 9Department of Gastroenterology and Hepatology, University Hospitals Leuven, KU Leuven, 10Department of Chronic Diseases, Metabolism and Ageing; Pneumology, KU Leuven, 11Department of Respiratory Diseases, University Hospitals Leuven

Capítulos

Resumen
Automatic Translation

This protocol describes rectal organoid morphology analysis (ROMA), a novel diagnostic assay for cystic fibrosis (CF). Morphological characteristics, namely the roundness (circularity index, CI) and the presence of a lumen (intensity ratio, IR), are a measure of CFTR function. Analysis of 189 subjects showed perfect discrimination between CF and non-CF.

Tags

Rectal Organoid Morphology Analysis ROMA Diagnostic Assay Cystic Fibrosis CFTR Mutations Sweat Fluoride Semi-automated Analysis Standardized Analysis Central Laboratory CFTR Modulatory Efficacy Restoration Of CFTR Function Organoid Staining Calcium Green Confocal Microscope Live Cell Imaging
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter