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Developmental Biology

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JoVE Journal Developmental Biology
FACS-isolatie en kweek van fibro-adipogene voorlopers en spierstamcellen van onverstoorbare en gewonde muizenskeletspieren
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FACS-isolatie en kweek van fibro-adipogene voorlopers en spierstamcellen van onverstoorbare en gewonde muizenskeletspieren

Article DOI: 10.3791/63983-v • 11:25 min • June 8th, 2022
June 8th, 2022 •
1, 2, 1
1Laboratory of Muscle Stem Cells and Gene Regulation, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH), 2Flow Cytometry Section, National Institute of Arthritis, Musculoskeletal, and Skin Diseases (NIAMS), National Institutes of Health (NIH)

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De precieze identificatie van fibro-adipogene voorlopercellen (FAPs) en spierstamcellen (MuSC's) is van cruciaal belang voor het bestuderen van hun biologische functie in fysiologische en pathologische omstandigheden. Dit protocol biedt richtlijnen voor de isolatie, zuivering en cultuur van FAPs en MuSC's van volwassen muisspieren.

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Ontwikkelingsbiologie spierfibrose-adipogene voorlopercellen spierstamcellen (MuSC's) mesenchymale stamcellen mesenchymale stromale cellen FACS spierregeneratie
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