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Tekniske anvendelser af mikroelektrodearray- og patch-klemmeoptagelser på humaninducerede pluripotente stamcelleafledte kardiomyocytter
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Tekniske anvendelser af mikroelektrodearray- og patch-klemmeoptagelser på humaninducerede pluripotente stamcelleafledte kardiomyocytter

Article DOI: 10.3791/64265-v • 10:30 min • August 4th, 2022
August 4th, 2022 •
1,2, 1,2, 1,2, 1,2, 1,2,3
1Stanford Cardiovascular Institute, Stanford University School of Medicine, 2Division of Cardiovascular Medicine, Department of Medicine, Stanford University School of Medicine, 3Department of Radiology, Stanford University School of Medicine

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Humaninducerede pluripotente stamcelleafledte kardiomyocytter (hiPSC-CM'er) er opstået som en lovende in vitro-model til lægemiddelinduceret kardiotoksicitetsscreening og sygdomsmodellering. Her beskriver vi en protokol til måling af kontraktilitet og elektrofysiologi af hiPSC-CM'er.

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