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JoVE Journal Biochemistry
Pulldown-analyse kombineret med co-ekspression i bakterieceller som et tidseffektivt værktøj til test af udfordrende protein-protein-interaktioner
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Journal (Biochemistry)
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Pulldown-analyse kombineret med co-ekspression i bakterieceller som et tidseffektivt værktøj til test af udfordrende protein-protein-interaktioner

Article DOI: 10.3791/64541-v • 07:03 min • December 23rd, 2022
December 23rd, 2022 •
1,2, 1, 1,2, 2, 1
1Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 2Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences

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Her beskriver vi en metode til bakteriel co-ekspression af differentielt mærkede proteiner ved hjælp af et sæt kompatible vektorer, efterfulgt af de konventionelle pulldown-teknikker til at studere proteinkomplekser, der ikke kan samles in vitro.

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Biokemi nr. 190
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