Name: Efecto similar al estrógeno de la cápsula de Bazi Bushen en ratas ovariectomizadas (Video) | JoVE Journal
Uploaded: 2023-04-07T14:40:00.000+00:00
Duration: 8 min 56 s
Description: Aprenda a evaluar los efectos similares al estrógeno de la cápsula Bazi Bushen en ratas ovariectomizadas mediante micro-CT e inmunohistoquímica. Este protocolo analiza la vía RANKL/OPG y el metabolismo óseo.

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<table><tbody><tr><td>10% de formalina tamponada neutra</td><td>Proteintech Group, Inc.</td><td>23408-1AP-100</td><td></td></tr><tr><td>Kits de ELISA de 17-Beta-Estradiol</td><td>Proteintech Group, Inc.</td><td>21933-1-AP</td><td></td></tr><tr><td>3%H2O2</td><td>ShanDong LIRCON Medical Technology Incorporated Company</td><td>20221027</td><td></td></tr><tr><td>Anticuerpo anti-osteoprotegerina</td><td>Abcam</td><td>ab203061</td><td></td></tr><tr><td>Cápsulas de Bazibushen</td><td>Shijiazhuang Yiling Pharmaceutical Co., Ltd.</td><td></td><td></td>XB2103001</tr><tr><td>Biotina cabra anti-conejo IgG</td><td>Abcam</td><td>ab207995</td><td></td></tr><tr><td>Kits de ensayo de calcio</td><td>Nanjing Jiancheng Instituto de Bioingeniería</td><td>C004-2-1</td><td></td></tr><tr><td>Diaminobencidina</td><td>ZSGB-BIO</td><td>ZLI-9018</td><td></td></tr><tr><td>Tabletas de valerato de estradiol</td><td>Bayer AG</td><td>156A</td><td></td></tr><tr><td>Gene 1580R centrífuga</td><td>GENE Co. Ltd.&nbsp;</td><td></td><td></td>GZ422515090077</tr><tr><td>Image-Pro Plus</td><td>Media Cybernetics</td><td>IPP 6.0</td><td></td></tr><tr><td>Microscopio DM6000B Leica (sistema de microscopio vertical totalmente automatizado)</td><td>Leica</td><td>361715</td><td></td></tr><tr><td>Solución de bloqueo de suero de cabra normal</td><td>Vector Laboratories</td><td>S-1000-20</td><td></td></tr><tr><td>Kits de ensayo de fosfato</td> Instituto de <td>Bioingeniería de Nanjing Jiancheng</td><td>C006-1-1</td><td></td></tr><tr><td>Solución salina tamponada con fosfato</td><td>Servicebio, Wuhan, China</td><td>CR10201M</td><td></td></tr><tr><td>Sistema de imágenes Quantum GX2 microCT</td><td>PerkinElmer</td><td>CLS149276</td><td></td></tr><tr><td>RANKL anticuerpo policlonal de conejo</td><td>Elabscience Biotechnology Co. Ltd.</td><td>E-EL-R3032-96T Rata</td><td></td></tr><tr><td>SOST (Esclerostina) Kit ELISA</td><td>Elabscience Biotechnology Co., Ltd.</td><td>E-EL-R1405c-96T Rata</td><td></td></tr><tr><td>TRACP-5b (Fosfatasa ácida resistente al tartrato 5b) Kit</td><td>ELISA Abcam</td><td>ab108667</td><td></td></tr><tr><td>Rata &beta;-CTx (Beta Crosslaps) Kit</td><td>ELISA Elabscience Biotechnology Co., Ltd.</td><td>E-EL-R0939C-96T</td><td></td></tr><tr><td>Anticuerpo policlonal de conejo esclerostina</td><td>Beijing Vital River Laboratory Animal Technology Co., Ltd.</td><td>SCXK(JING)2016-0006</td><td></td></tr><tr><td>Escáner de diapositivas</td><td>Hamamatsu Photonics K.K.</td><td>Nano Zoomer-SQ</td><td></td></tr><tr><td>Sprague Dawley ratas hembra Beijing</td><td>Vital River Laboratory Animal Technology Co., Ltd.</td><td>ZLI-9381</td><td></td></tr></tbody></table>

efecto similar al estrógeno de la cápsula de bazi bushen en ratas ovariectomizadas

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This study aims to show the estrogen-like effect of Bazi Bushen capsule (BZBS), a Chinese herbal compound, in ovariectomized mice. Female Sprague-Dawley ...

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Efecto similar al estrógeno de la cápsula de Bazi Bushen en ratas ovariectomizadas

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