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Увеличение продолжительности жизни нейтрофилов с помощью CLON-G и анализа спонтанной смерти in vitro
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Увеличение продолжительности жизни нейтрофилов с помощью CLON-G и анализа спонтанной смерти in vitro

Article DOI: 10.3791/65132-v • 05:52 min • May 12th, 2023
May 12th, 2023 •
*1,2, *3, *4, 1,2, 5, 1,2, 5
1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, 2Tianjin Institutes of Health Science, 3Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, 4Haihe Laboratory of Cell Ecosystem, Tianjin Medical University, 5Joint Program in Transfusion Medicine, Department of Pathology, Harvard Medical School; Division of Blood Bank, Department of Laboratory Medicine, The Stem Cell Program, Boston Children's Hospital, Dana-Farber /Harvard Cancer Center
* These authors contributed equally

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Этот протокол подробно описывает подготовку CLON-G для продления продолжительности жизни нейтрофилов до более чем 5 дней и обеспечивает надежную процедуру оценки гибели нейтрофилов с помощью проточной цитометрии и конфокальной флуоресцентной микроскопии.

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