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Bioengineering

Polydimethylsiloxanhaltigen Polycarbonat Mikrofluidiksysteme für Zellmigrationsstudien unter Perpendicular Chemische und Sauerstoff Gradients

Published: February 23, 2017 doi: 10.3791/55292
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1, 1, 1, 1, 1
1Research Center for Applied Sciences, Academia Sinica

Materials

Name Company Catalog Number Comments
1 ml Syringe Becton-Dickinson, Franklin Lakes, NJ 302104
1.5 ml Microcentrifuge Tube Smartgene 6011-000
10 ml Syringe Becton-Dickinson, Franklin Lakes, NJ 302151
15 ml Centrifuge Tube ThermoFisher Scientific,Waltham, MA Falcon 352096
150 mm Petri dish Dogger Science DP-43151
1H,1H,2H,2H-Perfluorooctyltrichlorosilane Alfa Aesar, Ward Hill, MA 78560-45-9
3 ml Syringe Becton-Dickinson, Franklin Lakes, NJ 302118
4'' Silicon Dummy Wafer Wollemi Technical, Taoyuan, Taiwan
Acetone ECHO Chemical, Miaoli, Taiwan AH3102-000000-72EC
AG Double Expose Mask Aligner M&R Nano Technology, Taoyuan, Taiwan AG500-4D-D-V-S-H
Antibiotic-Antimyotic solution ThermoFisher Scientific,Waltham, MA GIBCO 15240-062
Biopsy punch Miltex, Plainsboro, NJ 33-31
Blunt needle JensenGlobal, Santa Barbara, CA Gauge 14
Bright-Line Hemocytometer Sigma-Aldrich, St. Louis, MO Z359629 for cell counting
Buffered Oxide Etch ECHO Chemical, Miaoli, Taiwan PH3101-000000-72EC
Cell Culture Incubator Caron, Marietta, OH 6016-1
COMSOL Multiphysics COMSOL, Burlington, MA Ver. 4.3b for numerical simulation of chemical gradients in the device
D-PBS ThermoFisher Scientific,Waltham, MA GIBCO 14190-144
Desicattor A-VAC Industries, Anaheim, CA 35.10001.01
DMEM/F12+GlutaMax-1 ThermoFisher Scientific,Waltham, MA GIBCO 10565-018
Fetal Bovine Serum ThermoFisher Scientific,Waltham, MA GIBCO 10082
Fibronectin from Human Plasma Sigma-Aldrich, St. Louis, MO F2006
Inverted Fluorescence Microscope Leica Microsystems, Wetzlar, Germany DMIL LED
Isopropyl Alcohol (IPA) ECHO Chemical, Miaoli, Taiwan CMOS112-00000-72EC
JuLi Smart Fluorescence Cell Imager NanoEnTek, Seoul, Korea DBJ01B
Mechanical Convention Oven ThermoFisher Scientific,Waltham, MA Lindberg Blue M MO1450C
NaOH Showa Chemical Industry, Tokyo, Japan 1943-0150
Plasma tretment system Nordson MARCH, Concord CA PX-250 for oxygen plasma surface treatment
Polycarbonate (PC) film Quantum Beam Technologies, Tainan Taiwan
Polydimehtylsiloxane (PDMS) Dow Corning, Midland, MI SYLGARD 184
Pyrogallol Alfa Aesar, Ward Hill, MA A13405
Removable Adhesive Putty 3M 860
Sorvall Legend Mach 1.6R Tabletop Centrifuge ThermoFisher Scientific,Waltham, MA
Spin Coater ELS Technology, Hsinchu, Taiwan ELS 306MA
SU-8 2050 MicroChem, Westborough, MA SU-8 2050
SU-8 Developer MicroChem, Westborough, MA Y020100
Surgical blade Feather, Osaka, Japan 5005093 for PDMS cutting
Syringe Pump Chemyx, Houston, TX Fusion 400
T75 Cell Culture Flask ThermoFisher Scientific,Waltham, MA Nunc 156367
Trypan Blue Solution, 0.4% ThermoFisher Scientific,Waltham, MA 15250061
Trypsin-EDTA ThermoFisher Scientific,Waltham, MA GIBCO 25200
Tygon PTFE Tubing Saint-Gobain Performance Plastics, Akron, OH
Tygon Tubing Saint-Gobain Performance Plastics, Akron, OH 621

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References

  1. Phillips, J. E., Burns, K. L., Le Doux, J. M., Guldberg, R. E., García, A. J. Engineering graded tissue interfaces. Proc. Natl. Acad. Sci. USA. 105 (34), 12170-12175 (2008).
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  3. Oh, S. H., Ward, C. L., Atala, A., Yoo, J. J., Harrison, B. S. Oxygen generating scaffolds for enhancing engineering tissue survival. Biomaterials. 30 (5), 757-762 (2009).
  4. Decaris, M. L., Lee, C. I., Yoder, M. C., Tarantal, A. F., Leach, J. K. Influence of the oxygen microenvironment on the proangiogenic potential of human endothelial colony forming cells. Angiogenesis. 12, 303-311 (2009).
  5. Chung, B. G., et al. Human neural stem cell growth and differentiation in a gradient-generating microfluidic device. Lab Chip. 5 (4), 401-406 (2005).
  6. Harris, A. L. Hypoxia - a key regulatory factor in tumor growth. Nat. Rev. Cancer. 2 (1), 38-47 (2002).
  7. Allen, J. W., Bhatia, S. N. Formation of steady-state oxygen gradients in vitro: application to liver zonation. Biotechnol. Bioeng. 82 (3), 253-262 (2003).
  8. McCord, A. M., Jamal, M., Shankavaram, U. T., Lang, F. F., Camphausen, K., Tofilon, P. J. Physiologic oxygen concentration enhances the stem-like properties of CD133+ human glioblastoma cells in vitro. Mol. Cancer Res. 7 (4), 489-497 (2009).
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  10. Chang, C. W., et al. A polydimethylsiloxane-polycarbonate hybrid microfluidic device capable of generating perpendicular chemical and oxygen gradients for cell culture studies. Lab Chip. 14 (19), 3762-3772 (2014).
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  13. Xia, Y., Whitesides, G. M. Soft Lithography. Ann. Rev. Mate. Sci. 28, 153-184 (1998).
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  15. Cordelières, F. P. Manual Tracking. , Institute Curie. Orsay, France. https://imagej.nih.gov/ij/plugins/track/Manual%20Tracking%20plugin.pdf (2005).
  16. Asano, Y., Horn, E. Instructions Chemotaxis and Migration Tool 2.0. , ibidi GmbH. Martinsried, Germany. http://ibidi.com/fileadmin/products/software/chemotaxis_tool/IN_XXXXX_CT_Tool_2_0.pdf (2013).
  17. Chen, Y. H., Peng, C. C., Cheng, Y. J., Wu, J. G., Tung, Y. C. Generation of nitric oxide gradients in microfluidic devices for cell culture using spatially controlled chemical reactions. Biomicrofluidics. 7 (6), 064104 (2013).
Polydimethylsiloxanhaltigen Polycarbonat Mikrofluidiksysteme für Zellmigrationsstudien unter Perpendicular Chemische und Sauerstoff Gradients
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Cite this Article

Chiang, H. J., Yeh, S. L., Peng, C.… More

Chiang, H. J., Yeh, S. L., Peng, C. C., Liao, W. H., Tung, Y. C. Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients. J. Vis. Exp. (120), e55292, doi:10.3791/55292 (2017).

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