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Biology

有针对性的表达绿色荧光蛋白在头发上使用体外病毒转导的卵泡

Published: March 17, 2008 doi: 10.3791/708

ERRATUM NOTICE

Summary

毛囊中含有皮肤细胞类型的多样性是一个非常复杂的附属物。卵泡经过不断骑自行车通过有机体的生命,包括经济增长和增长依赖于特定的干细胞吸收。针对基因和干细胞的卵泡,以改变其属性,尤其是发干的性质,讨论。毛囊输送系统进行了描述,如脂质体和基因治疗的病毒载体。毛囊干细胞的性质进行了讨论,特别是它的全能性。

Abstract

毛囊中含有皮肤细胞类型的多样性是一个非常复杂的附属物。卵泡经过不断骑自行车通过有机体的生命,包括经济增长和增长依赖于特定的干细胞吸收。针对基因和干细胞的卵泡,以改变其属性,尤其是发干的性质,讨论。毛囊输送系统进行了描述,如脂质体和基因治疗的病毒载体。毛囊干细胞的性质进行了讨论,特别是它的全能性。

Protocol

在头发毛囊,使用前体内病毒transductionplease更多有针对性的表达GFP的信息,请访问斯普林格协议。

Tags

斯普林格协议,第13期,细胞生物学,毛囊,脂质体,腺病毒,基因,干细胞

Erratum

Formal Correction: Erratum: Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction
Posted by JoVE Editors on 03/07/2012. Citeable Link.

A correction was made to: Targeted expression of GFP in the hair follicle using ex vivo viral transduction. A revised abstract was republished due to a publisher error. The correct abstract is:

There are many cell types in the hair follicle, including hair matrix cells which form the hair shaft and stem cells which can initiate the hair shaft during early anagen, the growth phase of the hair cycle, as well as pluripotent stem cells that play a role in hair follicle growth but have the potential to differentiate to non-follicle cells such as neurons. These properties of the hair follicle are discussed. The various cell types of the hair follicle are potential targets for gene therapy. Gene delivery system for the hair follicle using viral vectors or liposomes for gene targeting to the various cell types in the hair follicle and the results obtained are also discussed.

This replaced:

The hair follicle is a highly complex appendage of the skin containing a multiplicity of cell types. The follicle undergoes constant cycling through the life of the organism including growth and resorption with growth dependent on specific stem cells. The targeting of the follicle by genes and stem cells to change its properties, in particular, the nature of the hair shaft is, discussed. Hair follicle delivery systems are described, such as liposomes and viral vectors for gene therapy. The nature of the hair follicle stem cells is discussed, in particular, its pluripotency.

有针对性的表达绿色荧光蛋白在头发上使用体外病毒转导的卵泡
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Cite this Article

Hoffman, R. M., Li, L. TargetedMore

Hoffman, R. M., Li, L. Targeted Expression of GFP in the Hair Follicle Using Ex Vivo Viral Transduction. J. Vis. Exp. (13), e708, doi:10.3791/708 (2008).

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