Single Cell Flow System
Single Cell Flow System - Related Videos
13:28
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry
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Cited by 33 •
2012
Microbial biofilms are generally constituted by distinct subpopulations of specialized cells. Single-cell analysis of these subpopulations requires the use of fluorescent reporters. Here we describe a protocol to visualize and monitor several subpopulationswithin B. subtilis biofilms using fluorescence microscopy and flow cytometry.
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
10:39
Single and Two-phase Flow in a Packed Bed Reactor
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2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA The goal of this experiment is to determine the magnitude of maldistribution in typical packed bed reactors in both single phase and two-phase (gas-liquid) flow and evaluate the effects of this maldistribution on pressure drop. The concepts of residence time distribution and dispersion are introduced through the use of tracers, and these concepts are related to physical...
19:26
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
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Cited by 30 •
2013
Intracellular Ca2+ dynamics are very important in sperm physiology and Ca2+-sensitive fluorescent dyes constitute a versatile tool to study them. Population experiments (fluorometry and stopped flow fluorometry) and single cell experiments (flow cytometry and single cell imaging) are used to track spatio-temporal [Ca2+] changes in human sperm cells.
08:09
Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
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Cited by 15 •
2013
The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.
FAQs
Controlled pressure or flow governs how individual cells move through microfluidic channels and enter defined measurement regions. This regulation helps establish a consistent relationship between cell position, movement, and the point at which signals are recorded. As a result, researchers can examine cells under controlled fluid-handling conditions rather than interpreting an undifferentiated bulk sample.
Microfluidic channels provide confined, defined paths that support the transport and isolation of individual cells. They also create organized regions where optical, electrical, or chemical signals can be measured in relation to a cell’s location. This spatial control helps connect a measured molecular property with the specific cell that produced it, which is essential when cellular behavior varies within a sample.
The system associates each recorded signal with the position and behavior of a particular cell as it moves through the measurement regions. Differences in optical, electrical, or chemical responses can therefore be examined at the single-cell level. This approach reveals cellular heterogeneity, including distinct biochemical states that may be averaged together and obscured when the same material is analyzed in bulk.
Depending on the measurement design, the platform can record optical, electrical, or chemical signals from cells passing through defined regions. These signal types provide different ways to associate cell behavior with molecular properties. In biochemical studies, the resulting measurements can support analysis of enzyme activity, signaling, and cellular responses to changing biochemical conditions.
A typical workflow transports cells through microfluidic channels using controlled pressure or flow, guides them to defined regions, and records signals from individual cells as they pass through. The resulting data are linked to cell position and behavior before being interpreted as molecular or biochemical measurements. This sequence supports analysis of cell-to-cell variation rather than only sample-level averages.
It is particularly useful when biochemical behavior differs among cells or when rare cell states may be hidden in bulk measurements. Researchers can apply the system to investigate enzyme activity, signaling, and responses to biochemical conditions at single-cell resolution. These measurements help clarify how molecular properties vary across cells and can improve the sensitivity of biochemical assays.
Single-cell measurements can expose rare cellular states, characterize heterogeneity, and improve assay sensitivity. By connecting measured molecular properties with cell behavior, the platform can also support more detailed interpretation of biochemical responses. Its applications include diagnostics, drug development, and systems biology, where understanding variation among individual cells can influence experimental conclusions and research decisions.