Sumo-trapping Protein
Sumo-trapping Protein - Related Videos
06:23
Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
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Cited by 3 •
2019
SUMO is an essential and highly conserved, small ubiquitin-like modifier protein. In this protocol we are describing the use of a stress-tolerant recombinant SUMO-trapping protein (kmUTAG) to visualize native, untagged SUMO conjugates and their localization in a variety of cell types.
13:39
Optical Trapping of Nanoparticles
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Cited by 7 •
2013
The following setup approach details low power optical trapping of dielectric nanoparticles using a double-nanohole in metal film.
01:41
Social Traps
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2020
Social traps are negative situations where people get caught in a direction or relationship that later proves to be unpleasant, with no easy way to back out of or avoid. The concept was orignally introduced by John Platt who applied psychology to Garrett Hardin's "Tragedy of the Commons", where in New England herd owners could let their cattle graze in the common ground. This situation seems like a good idea, but an individual could have an advantage. If they owned more cows, the larger...
11:45
Experimental Methods for Trapping Ions Using Microfabricated Surface Ion Traps
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Cited by 19 •
2017
This paper presents a microfabrication methodology for surface ion traps, as well as a detailed experimental procedure for trapping ytterbium ions in a room-temperature environment.
13:34
Gene Trapping Using Gal4 in Zebrafish
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Cited by 19 •
2013
This protocol describes the method of gene trap insertional mutagenesis using Gal4-VP16 as the primary reporter and GFP/RFP as secondary reporters in zebrafish. Approximately one in ten high-expressing F0 fish yield gene trap progeny co-expressing GFP and RFP. The screening procedure can be readily scaled to adapt to the size of the laboratory performing the insertional mutagenesis screen.
FAQs
Selectivity comes from a substrate-recognition surface derived from a SUMO protease or from a SUMO-interacting motif. The reagent retains the ability to bind SUMO conjugates but lacks catalytic activity, so it does not cleave the isopeptide bond connecting SUMO to the target protein. This preserves the modification during capture and supports downstream biochemical analysis.
A catalytically inactive trap prevents the captured SUMO conjugate from being processed during the experiment. That distinction matters because SUMOylation is reversible, and cleavage would remove the very modification being measured. Retaining the intact conjugate allows researchers to detect, purify, or identify modified proteins rather than measuring a mixture altered by the reagent itself.
The trap stabilizes interactions that might otherwise be difficult to observe in a biochemical sample. By selectively binding SUMO-modified proteins, it can preserve evidence of modification while extracts are analyzed. This is especially useful for examining SUMO-regulated pathways and responses associated with signals such as cellular stress or DNA damage.
Both designs use SUMO-recognition principles, but they derive binding from different molecular features. A protease-derived reagent uses the substrate-recognition surface while omitting catalytic activity, whereas a motif-based reagent relies on a SUMO-interacting motif. In either case, the intended outcome is selective binding to SUMO conjugates without cleavage of their linkage.
Researchers apply the reagent to cell or tissue extracts so SUMO-modified proteins can be selectively captured or enriched. The recovered material can then be examined by immunoblotting, further purified, or subjected to mass spectrometry. This workflow converts otherwise difficult-to-detect SUMO conjugates into an experimentally accessible fraction for biochemical characterization.
Enriched SUMOylated proteins can help identify modification targets and connect them with SUMO-regulated pathways. Comparing captured material from relevant cellular states can also support studies of responses to stress, DNA damage, and other signals described in the experimental system. The resulting protein information helps characterize which components are associated with reversible SUMO regulation.