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Biochemistry

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Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins
 

Localization of SUMO-modified Proteins Using Fluorescent Sumo-trapping Proteins

Article DOI: 10.3791/59576-v
April 27th, 2019

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Summary April 27th, 2019

SUMO is an essential and highly conserved, small ubiquitin-like modifier protein. In this protocol we are describing the use of a stress-tolerant recombinant SUMO-trapping protein (kmUTAG) to visualize native, untagged SUMO conjugates and their localization in a variety of cell types.

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