White Blood Cell Cytometry
White Blood Cell Cytometry - Related Videos
09:53
Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
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Cited by 4 •
2009
An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell loss.
04:57
A Flow Cytometry Assay to Identify Immune Cell Subsets in Peripheral Blood Mononuclear Cells
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2025
This video demonstrates a flow cytometry-based technique using two fluorochromes to identify CD4+ and CD8+ T cells, γδ T cells, B cells, natural killer (NK) cells, and monocytes in human peripheral blood mononuclear cells (PBMCs). Upon incubating the cells with fluorochrome-conjugated antibodies against the surface markers, the cell subsets are identified using an optimal flow cytometric gating strategy.
17:07
Flow Cytometry and Fluorescence-Activated Cell Sorting (FACS): Isolation of Splenic B Lymphocytes
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2023
Source: Perchet Thibaut1,2,3, Meunier Sylvain1,2,3, Sophie Novault4, Rachel Golub1,2,3 1 Unit for Lymphopoiesis, Department of Immunology, Pasteur Institute, Paris, France 2 INSERM U1223, Paris, France 3 Université Paris Diderot, Sorbonne Paris Cité, Cellule Pasteur, Paris, France 4 Flow Cytometry Platfrom, Cytometry and Biomarkers UtechS, Center for Translational Science, Pasteur Institute, Paris, France The overall function of the immune system is to defend the body against infectious...
10:43
Flow Cytometry Purification of Mouse Meiotic Cells
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Cited by 65 •
2011
An efficient method to obtain highly purified viable meiotic fractions from mouse testis is described, which combines a refined cell dissociation protocol with fluorescent activated cell sorting (FACS). This method takes advantage of differences in the DNA content and nuclear density of discrete meiotic fractions.
15:15
Flow Cytometry Analysis of Immune Cells Within Murine Aortas
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Cited by 87 •
2011
This paper presents a flow cytometry-based method to investigate the immune composition of aortas. The paper also illustrates an additional technique that allows examining surrounding adventitia and vessel wall separately. This method opens possibilities to perform phenotypical analyses of aortic leukocytes and apply several immunological assays for atherosclerosis studies.
FAQs
Laser-induced light scatter supplies physical information about leukocytes, including their relative size and internal complexity. Because cells are measured as they pass through the focused stream, the resulting signals can be converted into cellular profiles rather than treated as a single bulk measurement. This helps researchers compare immune cell populations according to structural features.
Labeled antibodies generate fluorescence signals that add molecular information to the physical measurements obtained from light scatter. These signals help characterize cellular identity and distinguish leukocyte populations with different molecular profiles. Combining antibody-associated fluorescence with scatter measurements supports immunophenotyping, allowing researchers to relate a cell’s molecular identity to its size and internal complexity.
Individual passage allows the cytometric system to associate detected scatter and fluorescence signals with particular cells. The instrument can therefore build cellular profiles from measurements made across many leukocytes instead of producing only an averaged sample-level signal. This single-cell basis supports quantitative assessment of abundance and identification of distinct immune cell populations.
Scatter and fluorescence provide complementary views of the same leukocyte population. Scatter contributes information about size and internal complexity, while fluorescence from labeled antibodies indicates molecular identity. Evaluating these signals together enables researchers to separate and characterize immune cell populations more meaningfully than relying on either physical or molecular measurements alone.
A typical measurement places the cell-containing sample into a system that forms a focused fluid stream. Leukocytes pass individually through the stream and encounter laser illumination, producing scatter and fluorescence signals. Detectors capture these signals, and the system converts them into cellular profiles that can be used for counting, immunophenotyping, and population identification.
In bioengineering studies, cytometry can connect leukocyte responses with exposure to an engineered biomaterial or therapeutic intervention. Measurements of cell abundance, physical characteristics, and molecular identity provide cellular evidence for comparing conditions. This makes the method useful for examining how designed materials or drugs influence immune-related populations during development and evaluation.
White blood cell cytometry supports cell-based product development by characterizing the cellular populations present in a product or experimental system. Quantitative measurements can describe abundance and distinguish populations using physical and molecular features. Such profiles help link the composition or response of cells to the design and evaluation of engineered cell-based products.
The method supplies quantitative cellular profiles that can be incorporated into immune monitoring strategies and diagnostic-system design. By measuring leukocyte abundance alongside size, internal complexity, and molecular identity, engineers can connect cellular patterns with a monitored condition or intervention. These outputs support development of systems intended to characterize immune responses in a structured, measurable way.