Opti-mem
Opti-mem - Related Videos
01:13
Five-Membered Heterocyclic Aromatic Compounds: Overview
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2023
Heterocyclic aromatic compounds are cyclic compounds that are aromatic and have one or more heteroatoms—atoms other than carbon, in the ring. Depending upon the number of atoms present in the ring, they can be either five or six-membered. Examples of five-membered heterocyclic aromatic compounds include pyrrole, furan, thiophene, and imidazole. Pyrrole consists of one nitrogen atom having one lone pair of electrons. Furan and thiophene have one oxygen and one sulfur heteroatom, respectively.
08:00
Quantitative Assessment of Immune Cells in the Injured Spinal Cord Tissue by Flow Cytometry: a Novel Use for a Cell Purification Method
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Cited by 33 •
2011
Quantification of cellular inflammation in the injured/pathological CNS by flow cytometry is complicated by lipid/myelin debris that can have similar size and granulation to cells, decreasing sensitivity/accuracy. We have advanced a cell preparation method to remove myelin debris and improve cell detection by flow cytometry in the injured spinal cord.
02:58
Generating Recombinant Live-Attenuated Influenza Viruses for Vaccines
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2025
The video showcases a method to create cold-adapted recombinant influenza viruses. It involves transfecting human cells with bi-directional plasmids, followed by incubation at lower temperature incubation to yield viruses suitable for vaccine production, characterized by reduced replication in warmer conditions.
03:38
A Method to Produce Recombinant Antibodies Against Metallopeptidase
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2025
This video demonstrates a procedure for the production of recombinant antibodies against metallopeptidase. Recombinant plasmids carrying genes for the antibody, green fluorescent protein, and antibiotic resistance are transfected into epithelial cells with lipofectamine. The transfected cells are selected and cultivated to generate recombinant antibodies.
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
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Cited by 129 •
2010
Rescue of influenza A viruses from plasmid DNA is a basic and essential experimental technique that allows influenza researchers to generate recombinant viruses to study multiple aspects in the biology of influenza virus, and to be used as potential vectors or vaccines.
FAQs
Opti-MEM’s low-protein environment can favor the formation and delivery of nucleic acid complexes during transfection. With less serum present, researchers can prepare these complexes in a more controlled setting. This is particularly relevant when plasmid DNA or RNA must reach mammalian cells for gene-expression experiments.
The balanced nutrients and salts in Opti-MEM help maintain mammalian cells while experiments are performed under reduced-serum conditions. That support matters because cells must remain suitable for manipulation, not merely survive handling. Maintaining this consistent environment helps researchers compare gene-expression experiments while limiting differences associated with serum content.
Reduced-serum conditions can reduce serum-related variability in cell-based assays, making the experimental environment more consistent across measurements. This is useful when differences in gene expression or cellular responses need to be attributed to the tested manipulation rather than changing serum contributions. Opti-MEM therefore supports cleaner comparisons during optimization and routine experimental work.
It can serve both as a medium for mammalian cell handling and as a preparation environment for nucleic acid delivery reagents. In plasmid DNA or RNA experiments, this links routine culture conditions with the manipulation step. Using one consistent medium across these activities can help standardize workflows and improve reproducibility.
Applications include routine mammalian cell handling, plasmid DNA transfection, RNA transfection, and optimization of gene-expression experiments. The medium is relevant whenever researchers need cells maintained in a consistent environment while introducing nucleic acids or evaluating expression outcomes. These uses connect day-to-day cell culture with targeted biological manipulation.
The main outcome is a more consistent experimental setting, rather than a guarantee of higher expression. By combining a balanced nutrient and salt environment with reduced serum exposure, Opti-MEM can help limit serum-related variation and support repeatable cell-based measurements. Researchers can use that consistency when interpreting transfection or gene-expression results.