Fitc-area Fluorescence
Fitc-area Fluorescence - Related Videos
01:58
Studying the Permeation of FITC and FITC-Ferritin through an In Vitro Blood-Brain Barrier Model
0 Views •
2025
This video demonstrates a method to study permeation through an in vitro blood-brain barrier (BBB) model using astrocytes and endothelial cells on a membrane insert, with free FITC and FITC-loaded ferritin. FITC-ferritin bound to receptors on endothelial cells and crossed the membrane barrier to the lower chamber while free FITC showed restricted permeation and remained in the upper chamber.
02:56
FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans
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2023
This video introduces a method of to visualize and measure the integrity of the C. elegans intestine lumen by feeding worms FITC-labeled dextran. The example protocol shows the assay done with 3,3’-diindolylmethane (DIM)-treated and pathogen-fed worms.
11:13
In Vitro Permeation of FITC-loaded Ferritins Across a Rat Blood-brain Barrier: a Model to Study the Delivery of Nanoformulated Molecules
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Cited by 15 •
2016
A method to establish an in vitro model of blood-brain barrier based on a co-culture of rat brain microvascular endothelial cells and astrocytes is described and validated. This system proved to be a valid tool to study the effect of nanoformulation on the trans-barrier permeation of fluorescent molecules.
04:50
Imaging FITC-dextran as a Reporter for Regulated Exocytosis
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Cited by 10 •
2018
Here we detail a method for live cell imaging of regulated exocytosis. This method utilizes FITC-dextran, which accumulates in lysosome-related organelles, as a reporter. This simple method also allows distinguishing between different modes of regulated exocytosis in cells that are difficult to manipulate genetically.
05:54
Intravital Fluorescence Microscopy to Study Microvascular Thrombus Formation
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2025
This video demonstrates the intravital fluorescence microscopy technique to study thrombus formation in a mouse model. The mouse is injected with a fluorophore-labeled polysaccharide to monitor blood circulation. Upon illumination with a high-intensity light, the phototoxic induction of an injury in the microvasculature of the ear leads to the formation of a thrombus, which is confirmed by visualizing the obstruction in the blood flow.
FAQs
The instrument records the FITC emission pulse generated as each labeled cell or particle crosses the focused excitation beam. It then integrates, or sums, the detected signal over the pulse duration to produce an area value. This calculation represents the accumulated fluorescence associated with that individual event rather than relying on one instantaneous measurement.
FITC-area fluorescence incorporates signal across the full duration of an emission pulse, so it is less dependent on brief fluctuations at the signal maximum. That makes the measurement useful when researchers compare fluorescence among cells or samples, because the integrated value captures the event’s total detected signal rather than only its highest point.
The measurement can support comparisons involving antigen expression, intracellular staining, and probe binding. In each case, FITC labels provide the detectable signal, while the integrated area supplies a quantitative value for individual biological particles. This allows researchers to examine differences in labeling-related fluorescence across cells or samples during quantitative cell biology studies.
The focused excitation beam stimulates fluorescence from FITC as a particle passes through the measurement region. Optical filters then direct the emitted green light toward the appropriate detector, which records the signal pulse. The resulting detector output supplies the intensity information that the instrument integrates to calculate the FITC-area value for that event.
A labeled cell or particle first passes through the focused excitation beam. FITC emits green fluorescence during that passage, and the optical system directs the emission through filters to a detector. The instrument records the resulting pulse and sums its intensity over time, generating an area measurement that can be used for comparisons among detected events.
Researchers can apply FITC-area fluorescence to immunophenotyping, assay development, and quantitative cell biology. It is particularly relevant when experiments compare antigen expression, intracellular staining, or probe binding across cells and samples. The measurement provides an integrated signal for labeled particles, supporting quantitative interpretation of fluorescence differences in these biological applications.