Lineage Tearing
Lineage Tearing - Related Videos
01:21
Lineage Commitment
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2023
Commitment is the process whereby stem cells: lose their ability to form all cell types and irreversibly change into a specific type. The multipotent hematopoietic stem cells, (HSCs), differentiate into the multipotent hematopoietic progenitor cells, (HPCs). The HPCs express many lineage-specific cytokine receptors. Each of these receptors binds specific cytokines, activates distinct signaling pathways, and expresses a particular gene set. The HPCs further differentiate to form committed...
04:38
A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome
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Cited by 4 •
2011
Capsulorhexis is an important step in phacoemulsification surgery. A surgeon creates a continous curvilinear tear on the anterior lens capsule by controlling the tearing vector forces. A peripherally extended tear is a serious complication. This video demonstrates a novel technique of rescuing capsular radial tear out using a cystotome.
07:45
Tear-Derived Exosomal miR-15a as New Diagnostic Tool for Diabetic Retinopathy
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Cited by 1 •
2025
This article describes a protocol for the collection and detection of miR-15a from tears as a new diagnostic tool for diabetic retinopathy.
09:22
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
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Cited by 12 •
2017
Analysis of tear film cytokines helps in studying various ocular diseases. Bead based multiplex assays are simple and sensitive and enable the testing of multiple targets in samples with small volumes. Here we describe a protocol for tear film cytokine profiling a using bead based multiplex assay.
07:35
Lineage Labeling of Zebrafish Cells with Laser Uncagable Fluorescein Dextran
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Cited by 8 •
2011
This protocol delineates a way to label and trace the fate of small groups of cells zebrafish embryos using UV-uncaging of caged fluorescein, followed by whole mount immunolabeling to amplify the signal from the uncaged fluorescein.
FAQs
Lineage tearing can arise when cells divide, migrate, or intercalate while tissues are being reshaped. These behaviors change which cells remain neighbors and where their descendants are positioned, even though the descendants retain a common progenitor. The resulting tissue can therefore contain regions whose cells share ancestry despite becoming separated during morphogenesis.
Final position reflects the cumulative effects of tissue movements, not ancestry alone. Cells that end up adjacent may come from different progenitors, while descendants of one progenitor may occupy distinct regions after rearrangement. Accounting for lineage tearing prevents researchers from treating spatial proximity as proof of common origin when interpreting developmental patterns.
It requires fate maps and clonal analyses to be read in two dimensions: where cells are located and which progenitor produced them. A clone distributed across separated regions may reflect tissue reorganization rather than unrelated origins. Conversely, neighboring cells with different ancestry may indicate that morphogenetic movements disrupted the original spatial arrangement.
Several behaviors can redistribute related descendants: coordinated cell divisions can expand a clone, migration can move cells across a developing tissue, and intercalation can insert cells between former neighbors. Together, these processes alter tissue geometry while preserving clonal relationships. Their contribution helps explain how a common ancestry can be associated with separated positions or regions.
Researchers should compare lineage information with the final spatial arrangement of cells rather than interpreting either record in isolation. Tracing clonal ancestry can identify shared progenitors, while examining tissue organization reveals how descendants were redistributed. This combined view helps distinguish genuine lineage relationships from patterns created by developmental rearrangement.
The concept is useful wherever tissues are built or reorganized through cell movements, including embryogenesis, regeneration, and disease-related tissue patterning. It links observable cell behavior with the later organization of organs and complex tissues. In these settings, recognizing torn lineage patterns can improve interpretation of how cells with shared ancestry contribute to different regions or fates.