Dimethyl Sulfoxide
Dimethyl Sulfoxide - Related Videos
02:35
Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide
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2025
Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.
13:59
Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
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Cited by 4 •
2013
Two methods for assigning the α- and ε-dimethylamine nuclear magnetic resonance signals of a reductively 13C-methylated N-terminal lysine are described. One method utilizes the pH-induced selectivity of the reductive methylation reaction, and the other uses aminopeptidase to selectively remove the N-terminal lysine.
11:53
Quantitative Proteomics Using Reductive Dimethylation for Stable Isotope Labeling
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Cited by 38 •
2014
Stable isotope labeling of peptides by reductive dimethylation (ReDi labeling) is a rapid, inexpensive strategy for accurate mass spectrometry-based quantitative proteomics. Here we demonstrate a robust method for preparation and analysis of protein mixtures using the ReDi approach that can be applied to nearly any sample type.
10:34
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
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Cited by 4 •
2022
The protocol provides instruction for modifying RNA with dimethyl sulfate for mutational profiling experiments. It includes in vitro and in vivo probing with two alternative library preparation methods.
10:09
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Cited by 2 •
2018
We present an effective and reproducible method to isolate and culture neural progenitor cells from embryonic and postnatal brain tissue for chromatin immunoprecipitation (ChIP) of histone 3 lysine 79 dimethylation (H3K79me2) - a histone mark located within the globular domain of histone 3.
FAQs
The sulfoxide group gives DMSO a highly polar structure, which supports strong interactions with ionic and polar substances. Because DMSO is aprotic, it can provide a polar reaction environment without behaving as a proton-donating solvent. This solvating ability can affect solvent-dependent reaction behavior, making the choice of DMSO relevant to how a chemical transformation proceeds.
Water miscibility allows DMSO to combine readily with aqueous systems, which is useful when polar compounds must remain in a shared liquid phase. Its relatively high boiling point supports its use in chemical operations where the solvent must remain liquid during reaction or processing. Together, these properties broaden its usefulness in reaction and extraction work.
DMSO can alter reaction behavior because its strong solvating ability stabilizes and surrounds dissolved ionic or polar substances. The resulting chemical environment may influence how reactants interact and how a transformation proceeds. This solvent-dependent effect is important in chemistry because the solvent is not merely a passive medium; its properties can contribute to the observed reaction outcome.
In transformations such as Swern oxidation, DMSO serves as an oxidant precursor rather than only as a reaction medium. Its sulfur-containing structure allows it to participate in the oxidation system under the appropriate reaction conditions. This illustrates why DMSO’s chemical role can extend beyond dissolving materials, particularly in synthetic chemistry where solvent and reagent functions may be connected.
DMSO can be selected as an extraction solvent when the target materials require a strongly polar environment for dissolution. Its ability to dissolve many ionic and polar substances, together with miscibility with water, makes it relevant to extraction systems involving polar components. The desired separation still depends on the substances present and on the solvent-dependent behavior of the system.
DMSO supports cryopreservation because it remains liquid at low temperatures and helps reduce ice-crystal damage to biological samples. Limiting this damage can help preserve sample integrity during low-temperature storage. In this context, its value arises from a physical property and a protective effect, rather than from its role as a reaction or extraction solvent.
DMSO is useful as a reaction solvent when strong solvating power, compatibility with polar or ionic substances, and a liquid medium that tolerates elevated temperatures are important. Its miscibility with water can also matter when aqueous components are involved. Chemists therefore consider DMSO for reactions whose behavior depends substantially on solvent polarity and composition.