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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing <em>In Vitro</em> and in Cells
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal Biochemistry
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

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10:34 min

December 09, 2022

DOI:

10:34 min
December 09, 2022

4025 Views
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Summary

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The protocol provides instruction for modifying RNA with dimethyl sulfate for mutational profiling experiments. It includes in vitro and in vivo probing with two alternative library preparation methods.

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