In Situ Synthesis
In Situ Synthesis - Related Videos
07:30
Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis
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Cited by 5 •
2018
In this work, a protocol for signal enhancement of nanoprobe-based biosensing is presented. The protocol is based on the reduction of chloroauric acid onto the surface of existing nanoprobes that consist of gold, silver, silica or iron-oxide nanoparticles.
08:33
Hydrogel Synthesis
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2023
Source: Amber N. Barron, Ashlea Patterson, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Hydrogels are a versatile class of cross-linked polymers produced through relatively simple procedures and with generally inexpensive materials. They can be formed from solution and involve a polymer backbone formed from monomer reagents, an initiator which makes the polymer reactive and a crosslinking species which binds the polymer chains...
08:00
Whole-Mount In Situ Hybridization
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2023
Whole-mount in situ hybridization (WMISH) is a common technique used for visualizing the location of expressed RNAs in embryos. In this process, synthetically produced RNA probes are first complementarily bound, or "hybridized," to the transcripts of target genes. Immunohistochemistry or fluorescence is then used to detect these RNA hybrids, revealing spatial and temporal patterns of gene expression. Unlike traditional in situ hybridization techniques, which require thin tissue sections whose...
09:42
Solid Phase Synthesis
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2023
Source: Vy M. Dong and Diane Le, Department of Chemistry, University of California, Irvine, CA Merrifield's solid-phase synthesis is a Nobel Prize winning invention where a reactant molecule is bound on a solid support and undergoes successive chemical reactions to form a desired compound. When the molecules are bound to a solid support, excess reagents and byproducts can be removed by washing away the impurities, while the target compound remains bound to the resin. Specifically, we will...
02:17
Synthesis and Decomposition Reactions
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2020
Synthesis and decomposition are two types of redox reactions. Synthesis means to make something, whereas decomposition means to break something. The reactions are accompanied by chemical and energy changes. Synthesis Reactions Synthesis reactions are also called combination reactions. It is a reaction in which two or more substances combine to form a complex substance. Synthesis reactions are generally represented as: A + B → AB or A + B → C. The formation of nitrogen dioxide is a synthesis...
FAQs
The key control points are the selected precursors and the conditions that allow them to combine into the desired intermediate, reagent, catalyst, or material. Because formation occurs within the working reaction mixture, the newly generated species can immediately encounter the next reactant or surrounding environment. This makes condition control important for directing the intended downstream chemistry.
Generating a species where it will be used can limit the need to isolate, transfer, and store it separately. That is especially useful when the intermediate or reagent is unstable or reactive, because fewer handling steps reduce its exposure outside the intended reaction environment. The approach therefore supports sequential chemistry while avoiding unnecessary manipulation of sensitive substances.
An isolated-compound workflow separates formation from later use, requiring the product to be recovered and transferred before subsequent chemistry. In situ synthesis connects those stages within one reaction environment. This can reduce purification and handling steps, although the desired species must still form under controlled conditions that allow it to participate effectively in the following reaction.
A typical workflow begins by selecting suitable precursors for the desired intermediate, reagent, catalyst, or material. Chemists then combine them in the reaction mixture or intended environment under controlled conditions. Once the target species forms, it is allowed to participate immediately in the next reaction or function in the surrounding system, avoiding a separate isolation and transfer stage.
Applications include catalyst generation, pharmaceutical research, materials research, coordination chemistry, and preparation of compounds whose properties depend on their local reaction environment. In each case, the method links formation with use, which can simplify multistep synthesis or preserve the conditions that influence the resulting compound, catalyst, or material during its intended chemical role.
By forming a needed species immediately before its next role, the approach can streamline multistep synthesis and reduce purification or handling between stages. It can also help when a compound’s properties depend on the local reaction environment, because formation and use occur in that setting. These features make it relevant to both synthetic efficiency and environment-dependent material preparation.