Calibration Plane

A calibration plane is the physical or electrical reference location at which an instrument’s measurements are defined, making it essential for accurate engineering testing. Calibration establishes this plane by measuring known standards, such as open, short, load, or through connections, and using the results to characterize and remove systematic errors introduced by cables, fixtures, and connectors. Engineers can then place the reference plane at the device under test or shift it mathematically through de-embedding and electrical-length correction. This concept is central to vector network analysis, impedance measurements, and high-frequency characterization, where small interconnect effects can significantly influence measured performance.

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Education

JoVE Science Education - Chemistry

Calibration Curves

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2023

Source: Laboratory of Dr. B. Jill Venton - University of Virginia Calibration curves are used to understand the instrumental response to an analyte and predict the concentration in an unknown sample. Generally, a set of standard samples are made at various concentrations with a range than includes the unknown of interest and the instrumental response at each concentration is recorded. For more accuracy and to understand the error, the response at each concentration can be repeated so an error...

Glassware Calibration

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2024

Accurate calibration of glassware, such as volumetric flasks, pipettes, and burettes, is essential to ensure accurate measurements in the analytical laboratory. Calibration helps maintain consistency across measurements and prevents errors arising from inaccurate volumes. Volumetric flasks: Volumetric flasks are designed to prepare aqueous solutions of precise volumes accurately with a calibration line on the neck. To calibrate a volumetric flask, it is important to fill it with distilled...

Pressure Transducer: Calibration Using a Pitot-static Tube

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2023

Source: Shreyas Narsipur, Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC Fluid pressure is an important flow characteristic that is required to determine the aerodynamics of a system. One of the oldest and still existing pressure measurement systems is the manometer due to its accuracy and simplicity of operation. The manometer is generally a U-shaped glass tube that is partially filled with liquid, as shown in Figure 1. The U-tube manometer requires no...

Instrument Calibration

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2024

Instrument calibration is essential for ensuring that instruments produce accurate and consistent results. It is vital in manufacturing, healthcare, testing laboratories, and scientific research. Calibration processes are specific to each instrument and help enhance data accuracy. Each instrument has a unique calibration process tailored to its design and function to improve data accuracy. Analytical Balance Calibration An analytical balance measures mass and requires regular calibration to...

Research

JoVE Journal - Chemistry

Determining the Ice-binding Planes of Antifreeze Proteins by Fluorescence-based Ice Plane Affinity

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Cited by 48 •

2014

Antifreeze proteins (AFPs) bind to specific planes of ice to prevent or slow ice growth. Fluorescence-based ice plane affinity (FIPA) analysis is a modification of the original ice-etching method for determination of AFP-bound ice planes. AFPs are fluorescently labeled, incorporated into macroscopic single ice crystals, and visualized under UV light.

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