Plant Freezing

Plant freezing is the exposure of plants or plant tissues to subzero temperatures, a form of cold stress that can limit growth, survival, and ecosystem distribution. As temperatures fall below the freezing point, ice typically forms in extracellular spaces, drawing water from cells and causing dehydration, membrane damage, and metabolic disruption; prior cold acclimation can improve tolerance by altering cellular solutes and protective proteins. Studying plant freezing helps explain frost injury, seasonal adaptation, and species distribution in changing environments. This knowledge also supports crop breeding, frost-protection strategies, seed and tissue preservation, and predictions of plant responses to climate variability.

Plant Freezing - Related Videos

Research

JoVE Journal - Biology

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

0 Views •

Cited by 20 •

2014

Obtaining high-quality transmission electron microscopy images is challenging, especially in the case of plant cells, which have abundant large water-filled vacuoles and aerated spaces. Tandem high-pressure freezing and quick freeze substitution greatly reduce preparation time of plant samples for TEM while producing samples with excellent ultrastructural preservation.

Protocol for Producing Three-Dimensional Infrared Video of Freezing in Plants

0 Views •

Cited by 1 •

2018

Here, we present a protocol to image a strawberry plant freezing in 3 dimensions. Two infrared cameras positioned at slightly different angles are used to produce a red-blue anaglyph video to observe the freezing of the plant in 3 dimensions.

Fundamental Technical Elements of Freeze-fracture/Freeze-etch in Biological Electron Microscopy

0 Views •

Cited by 6 •

2014

Basic techniques and refinements of freeze-fracture processing of biological specimens and nanomaterials for examination by transmission electron microscopy are described. This technique is a preferred method for revealing ultrastructural features and specializations of biological membranes and for obtaining ultrastructural level dimensional and spatial data in materials sciences and nanotechnology products.

An Easy Method for Plant Polysome Profiling

0 Views •

Cited by 20 •

2016

This protocol describes an easy method to extract and fractionate transcripts from plant tissues on the basis of the number of bound ribosomes. It allows a global estimate of translation activity and the determination of the translational status of specific mRNAs.

Research

JoVE Journal - Biology
Free Sample

Transmitting Plant Viruses Using Whiteflies

0 Views •

Cited by 41 •

2013

Improving our understanding of and our ability to manage many of the insect-transmitted plant viruses requires the use of the vector. Insect transmission of plant viruses is a tritrophic interaction, and as such requires the manipulation of insects, virus, and plant. Vectors must be reared in large numbers and manipulated in such a way as to insure high rates of transmission to test plants. The basics of rearing and manipulating the whitefly, Bemisa tabaci (Hemiptera: Aleyrodidae), a vector of...

View All Results

FAQs

Related Topics