Fluorescent Target Cells
Fluorescent Target Cells - Related Videos
12:09
The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
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Cited by 12 •
2014
The ability to monitor T cell responses in detail in vivo is important for the development of our understanding of the immune response. Here we describe the use of fluorescent target arrays (FTAs) in an in vivo T cell assay that assesses >250 parameters simultaneously by flow cytometry.
08:29
Workflow Using a Cryogenic Coincident Fluorescence, Electron, and Ion Beam Microscope for Targeted Milling of Cells
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Cited by 2 •
2025
This workflow enables lamella production targeting fluorescently labeled biological structures that are small (<1 μm in axial extent) and rare (1 copy per cell) using a cryogenic tri-coincident imaging platform. This platform integrates fluorescence microscopy, focused ion beam milling, and scanning electron microscopy at a single focal position and enables simultaneous fluorescence microscopy while milling.
10:36
In Vitro Imaging and Quantification of the Drug Targeting Efficiency of Fluorescently Labeled GnRH Analogues
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Cited by 1 •
2017
Selectively labeled fluorescent GnRH-I, -II and -III derivatives are reliable tools for tracking and quantifying their cellular uptake. This manuscript introduces experiments to visualize, quantify and compare the uptake efficiency of these GnRH conjugates in various cell lines.
09:24
Molecular Imaging to Target Transplanted Muscle Progenitor Cells
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Cited by 3 •
2013
A non-invasive means to evaluate the success of myoblast transplantation is described. The method takes advantage of a unified fusion reporter gene composed of genes whose expression can be imaged with different imaging modalities. Here, we make use of a fluc reporter gene sequence to target cells via bioluminescence imaging.
12:32
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
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Cited by 14 •
2013
Retrograde transport of fluorescent dye labels a sub-population of neurons based on anatomical projection. Labeled axons can be visually targeted in vivo, permitting extracellular recording from identified axons. This technique facilitates recording when neurons cannot be labeled through genetic manipulation or are difficult to isolate using 'blind' in vivo approaches.
FAQs
A fluorophore attached to the target cells absorbs excitation light and emits detectable light at a different wavelength. This signal creates a measurable distinction between labeled targets and unlabeled lymphocytes, allowing researchers to identify the two cell populations during microscopy or flow cytometry. The separation is essential for attributing changes specifically to target-cell fate.
Changes in membrane integrity and morphology provide evidence about how target cells respond during contact with immune effector cells. Microscopy can reveal visible cellular alterations, while flow cytometry can quantify fluorescent populations and associated changes. Together, these readouts help assess whether experimental conditions affect target-cell survival or produce measurable signs of cell damage.
A change in the number of detectable fluorescent target cells can indicate altered target-cell survival during an immune interaction. In cytotoxicity assays, this measurement supports quantitative evaluation of natural killer cell or cytotoxic T-cell activity. Comparing the target-cell signal across experimental conditions helps researchers determine whether immune responses differ in their effects on cellular fate.
Researchers expose labeled target cells to immune effector cells and then measure target-cell outcomes using microscopy or flow cytometry. The resulting changes in cell number, morphology, or membrane integrity provide a readout of cell-mediated killing. This design allows natural killer cell and cytotoxic T-cell activities to be evaluated and compared under defined experimental conditions.
Microscopy and flow cytometry are the principal measurement approaches described for these labeled cells. Microscopy supports observation of target-cell morphology and interactions, whereas flow cytometry provides detection and measurement of fluorescently distinguished populations. Selecting between them depends on whether the experiment emphasizes visual cellular changes, quantitative population analysis, or both types of information.
In immunology, they enable cytotoxicity assays that measure how immune effector cells affect targets. In infection research, the targets can represent pathogen-infected cells, allowing investigators to monitor immune responses against infected cellular populations. Fluorescent labeling also supports comparisons across experimental conditions, helping connect immune activity with target-cell number, morphology, membrane integrity, and fate.