Gas Permeable Chambers
Gas Permeable Chambers - Related Videos
11:48
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
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Cited by 10 •
2010
An Introduction into the technology, protocol and handling of the Corning HYPERStack Vessels and accessories used for high yield adherent cell culture. The protocol will show how to use the closed system vessels for increasing cell harvesting over current stacked plate products.
11:11
Canine Intestinal Organoids in a Dual-Chamber Permeable Support System
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Cited by 18 •
2022
Here, we present a protocol describing the culture of canine intestinal organoids in a dual-chamber, permeable support system. Organoid seeding in the permeable supports, the monolayer maintenance, and subsequent drug permeability experiments are described.
11:50
Measurement of Greenhouse Gas Flux from Agricultural Soils Using Static Chambers
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Cited by 143 •
2014
This article showcases the static chamber-based method for measurement of greenhouse gas flux from soil systems. With relatively modest infrastructure investments, measurements may be obtained from multiple treatments/locations and over timeframes ranging from hours to years.
08:42
In Situ Gas Analysis and Fire Characterization of Lithium-Ion Cells During Thermal Runaway Using an Environmental Chamber
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Cited by 3 •
2023
Here, we describe a test procedure developed to characterize thermal runaway and fires in lithium-ion cells through in situ measurements of various parameters in an environmental chamber.
07:51
Gas Absorber
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2023
Source: Michael G. Benton and Kerry M. Dooley, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA Gas absorbers are used to remove contaminants from gas streams. Multiple designs are used to accomplish this objective1. A packed bed column uses gas and liquid streams running counter to each other in a column packed with loose packing materials, such as ceramics, metals, and plastics, or structured packing1. The packed bed uses surface area created by the packing to...
FAQs
Gas exchange occurs by diffusion through the chamber’s gas-permeable membrane or material. This boundary allows oxygen and other gases from the surrounding atmosphere to reach the enclosed biological system while retaining liquid contents and cells, tissues, or microorganisms. Researchers can therefore modify the external gas environment while preserving the specimen and its experimental surroundings.
Gas conditions can shape biological responses, particularly when oxygen availability changes. By controlling exposure around an enclosed specimen, researchers can examine how oxygen-limited or shifting environments affect immune-cell function, pathogen survival, and interactions between host and pathogen. The chamber helps separate gas-related effects from disturbances that could result from repeatedly opening the system.
A gas permeable chamber permits gas exchange across its boundary without requiring repeated access to the contents. This preserves the liquid environment and enclosed biological material while the surrounding atmosphere is adjusted. In contrast, repeatedly opening a system can interrupt the controlled enclosure. The distinction is especially relevant when studying responses to sustained or changing gas exposure.
Oxygen availability is a central variable because infection and inflammation can create oxygen-limited environments. Gas permeable chambers allow researchers to adjust oxygen exposure around cells, tissues, or microorganisms and then assess associated biological responses. This supports comparisons of pathogen survival, immune-cell activity, and host-pathogen interactions under differing oxygen conditions.
A general workflow places the selected cells, tissue, or microorganisms within the chamber while retaining the relevant liquid contents. The chamber is then exposed to a defined surrounding atmosphere, allowing gases to diffuse through the boundary without repeatedly opening the enclosure. Researchers can subsequently examine how the selected gas conditions influence the biological system.
They are useful when researchers need to model oxygen-limited or changing environments associated with inflammation, tissue infection, or microbial growth. The approach can support studies of pathogen survival, immune-cell function, and host-pathogen interactions. It is particularly relevant when maintaining the specimen in a liquid-containing enclosure while varying the surrounding gas exposure.