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Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
JoVE Journal
Biology
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JoVE Journal Biology
Closed System Cell Culture Protocol Using HYPERStack Vessels with Gas Permeable Material Technology
DOI:

11:48 min

November 29, 2010

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Chapters

  • 00:06Title
  • 01:06HYPERStack Vessel Component Summary
  • 02:44Vessel Preparation
  • 05:34Isolation Procedure to Lock Liquid into the Layers
  • 07:09Harvest Protocol
  • 10:37Gas Diffusion Results through Gas Permeable Film used in the HYPERStack Vessels
  • 11:31Conclusion

Summary

Automatic Translation

An Introduction into the technology, protocol and handling of the Corning HYPERStack Vessels and accessories used for high yield adherent cell culture. The protocol will show how to use the closed system vessels for increasing cell harvesting over current stacked plate products.

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