Cd146 Affinity Column
Cd146 Affinity Column - Related Videos
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Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
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Cited by 3 •
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This protocol describes an isolation technique for obtaining primary lung resident mesenchymal stromal cells from rats, through the use of enzymatic digestion, density gradient separation, plastic adherence and CD146+ magnetic bead selection.
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Column Chromatography - Concepts
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Column Chromatography Multiple techniques exist to purify and separate compounds in an organic chemistry laboratory. One of the most reliable separation techniques for microscale separations, where less than 10 grams of the compound is available for analysis, is column chromatography. This technique separates compounds in a mixture based on their physical properties, such as their solubility, polarity, hydrophobicity, size, and charge. Chromatography Basics The main components of any...
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Column Chromatography - Student Protocol
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Source: Lara Al Hariri and Ahmed Basabrain at the University of Massachusetts Amherst, MA, USA Column Chromatography of Food DyeExpand In this experiment, you'll use column chromatography to separate green food coloring into its component blue and yellow dyes, called erioglaucine and tartrazine. First, you'll use 95% ethanol to elute the dye with a weaker affinity for silica gel. Then, you'll flush the second dye from the column with water. To recover the purified dyes, you will collect each...
09:23
Column Chromatography
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Source: Laboratory of Dr. Jimmy Franco - Merrimack College Column chromatography is one of the most useful techniques for purifying compounds. This technique utilizes a stationary phase, which is packed in a column, and a mobile phase that passes through the column. This technique exploits the differences in polarity between compounds, allowing the molecules to be facilely separated.1 The two most common stationary phases for column chromatography are silica gel (SiO2) and alumina (Al2O3), with...
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Electron Affinity
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2020
The electron affinity (EA) is the energy change for adding an electron to a gaseous atom to form an anion (negative ion). This process can be either endothermic or exothermic, depending on the element. Many of these elements have negative values of EA, which means that energy is released when the gaseous atom accepts an electron. However, for some elements, energy is required for the atom to become negatively charged, and the value of their EA is positive. Just as with ionization energy,...
FAQs
Selectivity is primarily established by the immobilized ligand: an antibody or other binding partner must interact specifically with CD146 or the intended binder. During sample passage, these interactions retain relevant molecules on the column while unrelated components remain in the flow-through. This separation is valuable when biological samples contain many different molecules and downstream work requires greater specificity.
The ligand provides the molecular recognition site that distinguishes CD146-associated material from unrelated sample components. Its identity, whether an antibody or another binding partner, determines which molecules can interact with the column and become retained. This makes ligand selection central to obtaining a preparation suited to protein purification, antibody purification, or characterization of CD146-related material.
Recovery occurs through controlled changes in buffer conditions that weaken or disrupt the interaction between the immobilized ligand and its bound material. The released fraction can then be collected separately from components that passed through earlier. Because elution is controlled rather than simply collected with the initial flow-through, the procedure helps distinguish retained CD146-related material from unbound sample constituents.
Selective capture concentrates CD146 protein or molecules that specifically bind it from a complex biological sample. The resulting material can support biomarker characterization and studies of endothelial, vascular, and tumor-associated biology. By reducing unrelated components, the column can improve sample specificity and provide more suitable reagents for diagnostic and therapeutic research.
A typical workflow begins by passing the complex biological sample through a column containing an immobilized CD146 ligand. Relevant molecules interact with that ligand and remain retained, whereas unrelated components pass through. Controlled buffer changes then release the bound material, allowing the recovered fraction to be examined or used for purification and subsequent medical research.
The essential components are a chromatography column, an immobilized CD146 ligand such as an antibody or binding partner, a complex biological sample, and buffers that can be changed in a controlled manner. The ligand provides selective recognition, while buffer manipulation governs release. Together, these components determine whether the desired material is retained and subsequently recovered.
This approach is relevant when investigators need CD146 protein or antibodies for focused analysis rather than an unfractionated biological sample. It can support biomarker characterization and research into endothelial, vascular, and tumor-associated biology. The technique is also useful when diagnostic or therapeutic studies require reagents with improved specificity for CD146-related investigations.
The recovered fraction can provide enriched CD146 protein or molecules that specifically bind CD146, creating material for further characterization. Researchers may use it to examine biomarker-related properties or to generate reagents for diagnostic and therapeutic research. Interpretation should distinguish this bound fraction from the flow-through, which contains components that did not interact with the immobilized ligand.