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Endogenous Staining of ATG9A from HEK293A Cells (Video) | JoVE
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Endogenous Staining of ATG9A from HEK293A Cells (Video) | JoVE

Investigating ATG9A Localization in Cells

Article DOI: 10.3791/200171-v • 03:19 min • June 16th, 2023
June 16th, 2023 •
*1,2, *1, *1, 1
1Molecular Cell Biology of Autophagy, The Francis Crick Institute, 2MRC Laboratory of Molecular Biology
* These authors contributed equally

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ATG9A Lipid-protein Interaction Vesicle Trafficking Autophagy Pathway Phagophore Formation Organelle Homeostasis Protein Machineries Protein Engineering AlphaFold In-situ Cryo-EM Autophagosomes Formation Protein Tags N-terminus C-terminus Drawbacks Detection Workflow Quantifying ATG9A Dispersal Immunofluorescence Live Imaging Image Acquisition And Analysis Cellular Compartments Standardization Of Pipelines Reliability And Reproducibility Of Results
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