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Immunology and Infection

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JoVE Journal Immunology and Infection
用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测
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用于适应高通量/高含量筛查的细胞内分枝杆菌定量的微观表型检测

Article DOI: 10.3791/51114-v • 15:28 min • January 17th, 2014
January 17th, 2014 •
*1, *1, *1, 1, 1, 1, 1, 1, 1
1Inserm U1019 - CNRS UMR 8024, Institut Pasteur de Lille, Université de Lille
* These authors contributed equally

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在这里，我们描述了一种表型检测，适用于小干扰合成RNA（siRNA）、化合物和 结核 分枝杆菌突变库的高通量/高含量屏幕。该方法依靠使用自动共聚焦显微镜检测荧光标记的荧光结 核分枝杆菌 。

Tags

免疫学和感染学，第83期， 结核分枝杆菌，高含量/高通量筛查，化疗基因组学，药物发现，siRNA库，自动共焦显微镜，基于图像的分析
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