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Face Detection En de óxido nítrico e superóxido na camada endotelial das artérias intactas

Article DOI: 10.3791/53718-v • 08:58 min • February 25th, 2016
February 25th, 2016 •
1,2, 1,2, 2,3, 1,2, 1,2
1Cardiovascular and Aging Research, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg, 2Kidney Control of Homeostasis, Swiss National Centre of Competence in Research, 3System Physiology, Department of Medicine, Division of Physiology, Faculty of Science, University of Fribourg

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Neste artigo descrevemos um método relativamente fácil adaptados usando o corante de fluorescência diaminofluorescein-2 diacetato (DAF-2DA) e dihydroethidium (DHE) para en face detecção simultânea e visualização de óxido nítrico intracelular (NO) e ânion superóxido (O 2 .- ), respectivamente, em aortas isoladas de fresco intactas de um modelo de rato de obesidade.

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Biologia Molecular Edição 108 Artérias microscopia confocal DHE DAF-2DA endotélio o óxido nítrico ânion superóxido Biologia Vascular
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