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JoVE Journal Neuroscience
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Bestemmelse af celleoverfladeekspression og endocytisk proteinhastighed i primære astrocytkulturer ved anvendelse af biotinylering

Article DOI: 10.3791/55974-v • 10:42 min • July 3rd, 2017
July 3rd, 2017 •
1, 1
1Department of Cellular and Physiological Sciences, University of British Columbia

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To biotinyleringsbaserede metoder, der er designet til bestemmelse af celleoverfladeekspression og endocytisk hastighed af proteiner udtrykt i plasmamembranen, er præsenteret i denne rapport.

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Neurovidenskab udgave 125 celleoverfladeekspression endocytose biotinylering astrocytter aquaporin-4 plasmamembran
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