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Cancer Research

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Preparation of Exosomes for siRNA Delivery to Cancer Cells

Article DOI: 10.3791/58814-v • 09:59 min • December 5th, 2018
December 5th, 2018 •
*1, *1, 1
1Institute of Pharmaceutical Science, King's College London
* These authors contributed equally

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An exosome is a new generation of drug delivery carriers. We established an exosome isolation protocol with high yield and purity for siRNA delivery. We also encapsulated fluorescently labelled non-specific siRNA into exosomes and investigated the cellular uptake of siRNA-loaded exosomes in cancer cells.

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Exosome Preparation SiRNA Delivery Cancer Cells Conditioned Medium Bioreactor Flasks Sucrose Cushion Ultracentrifugation Contaminating Proteins Non-exosomal Vesicles Sucrose Gradient Deuterium Oxide In Vitro Delivery RNA Interference-based Therapy Pancreatic Cancer HEK-293 Cells T75 Flasks Medium Reservoir Exosome-depleted Medium
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