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JoVE Journal Biology
Forbedret Cross Linking Immunoprecipitation (eCLIP) metode til effektiv identifikation af protein-bundet RNA i Mouse testis
A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
Journal (Biology)
A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
Membrane Puncture of Human Embryonic Kidney Cells and Giant Unilamellar Vesicles Using Gold Nanoparticles (Video) | JoVE
Journal (Biology)
Membrane Puncture of Human Embryonic Kidney Cells and Giant Unilamellar Vesicles Using Gold Nanoparticles (Video) | JoVE
 
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Forbedret Cross Linking Immunoprecipitation (eCLIP) metode til effektiv identifikation af protein-bundet RNA i Mouse testis

Article DOI: 10.3791/59681-v • 10:31 min • May 10th, 2019
May 10th, 2019 •
*1, *1, *1, *1, 1, 1, 1, 1, 1
1State Key Laboratory of Reproductive Medicine, Nanjing Medical University
* These authors contributed equally

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Her præsenterer vi en eCLIP-protokol for at bestemme store RNA-mål for RBP-kandidater i testikler.

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Biologi CLIP eCLIP UV Cross Linking ikke-radioaktivt mus testis RNA-bindende proteiner (RBPs)
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