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Immunofluorescence Labelling of Human and Murine Neutrophil Extracellular Traps in Paraffin-Embedded Tissue

Article DOI: 10.3791/60115-v • 07:17 min • September 10th, 2019
September 10th, 2019 •
1,2, 1
1Microscopy Core Facility, Max Planck Institute for Infection Biology, 2Cellular Microbiology, Max Planck Institute for Infection Biology

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Neutrophil extracellular traps (NETs) are three-dimensional structures generated by stimulated neutrophil granulocytes. It has become clear in recent years that NETs are involved in a wide variety of diseases. Detection of NETs in tissue may have diagnostic relevance, so standardized protocols for labelling NET components are required.

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Immunofluorescence Labelling Human Neutrophil Extracellular Traps Murine Neutrophil Extracellular Traps Paraffin-embedded Tissue Heat-induced Antigen Retrieval Protocol Neutrophil Elastase Antigen High PH Value Histones NETs Paraffin Tissues Mouse Men Archived Material Retrospective Study Water Bath Heat-induced Epitope Retrieval Buffer Glycerol Slides Incubate Room Temperature Ionized Water TBS Hydrophobic Barrier Pen Blocking Buffer
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