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Neuroscience

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JoVE Journal Neuroscience
Laser Capture Microdissection af Glioma subregions for rumlige og molekylære karakterisering af intratumoral heterogenitet, Oncostreams, og Invasion
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Laser Capture Microdissection af Glioma subregions for rumlige og molekylære karakterisering af intratumoral heterogenitet, Oncostreams, og Invasion

Article DOI: 10.3791/60939-v • 09:09 min • April 12th, 2020
April 12th, 2020 •
1,2,4, 1,2,4, 1,3, 1,2,4, 3, 1,2,4, 1,2,4
1Dept. of Neurosurgery, University of Michigan Medical School, 2Dept. of Cell and Developmental Biology, University of Michigan Medical School, 3Dept. of Ophthalmology & Visual Science, University of Michigan Medical School, 4Rogel Cancer Center, University of Michigan Medical School

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Laser microdissection (LMD) er en følsom og meget reproducerbar teknik, der kan bruges til at afdække veje, der mægle gliom heterogenitet og invasion. Her beskriver vi en optimeret protokol til at isolere diskrete områder fra gliomvæv ved hjælp af laser LMD efterfulgt af transskriptionsanalyse.

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Neurovidenskab gliom laser mikrodissection transskriptionomisk analyse RNA integritet tumor heterogenitet invasion molekylære mål
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