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Visualisering av svarande möten med en antigenmärkt blockerande lesion

Article DOI: 10.3791/61689-v • 08:24 min • July 27th, 2021
July 27th, 2021 •
*1, *2, 1, 3, 1, 4, 5, 6, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, 3Department of Molecular Biology and Genetics, Cornell University, 4Department of Cellular and Molecular Medicine, University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
* These authors contributed equally

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Medan replikationsgaffelkollisioner med DNA-addukter kan inducera dubbelsträngsbrott, är mindre känt om interaktionen mellan replisomer och blockerande lesioner. Vi har använt närhetsligeringsanalysen för att visualisera dessa möten och för att karakterisera konsekvenserna för svarande komposition.

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Biologi utgåva 173 svarande blockerande lesion PLA närhetsligeringsanalys DNA-interstrandtvärbindningar antigenmärkta tvärbindningar
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