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Journal / Biochemistry / Derivatisering av Protein kristaller med I3C med…
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Biochemistry
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JoVE Journal Biochemistry
Derivatisering av Protein kristaller med I3C med hjälp av Random Microseed Matrix Screening
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High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits
Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis (Video) | JoVE
Journal (Biochemistry)
Preparation of Dried Extracellular Vesicle (EV) Sample for Proteomics and Phosphoproteomics Analysis (Video) | JoVE
 
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Derivatisering av Protein kristaller med I3C med hjälp av Random Microseed Matrix Screening

Article DOI: 10.3791/61894-v • 14:04 min • January 16th, 2021
January 16th, 2021 •
*1, *1,2, 1,2, 1
1School of Biological Sciences, The University of Adelaide, 2Institute of Photonics and Advanced Sensing (IPAS), School of Biological Sciences, The University of Adelaide
* These authors contributed equally

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Denna artikel presenterar en metod för att generera proteinkristaller derivatized med I3C (5-amino-2,4,6-triiodoisophthalic syra) med hjälp av microseeding att generera nya kristallisation villkor i gles matris skärmar. Brickorna kan ställas in med hjälp av flytande dispenseringsrobotar eller för hand.

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Biokemi seedning slumpmässig mikroseed matris screening rMMS proteinkristallografi fasning Magic Triangle I3C
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