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JoVE Journal Behavior
Embryomikroinjektion og knockout-mutant identifikation af CRISPR/Cas9-genomredigeret Helicoverpa Armigera (Hübner)
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Embryomikroinjektion og knockout-mutant identifikation af CRISPR/Cas9-genomredigeret Helicoverpa Armigera (Hübner)

Article DOI: 10.3791/62068-v • 06:37 min • July 1st, 2021
July 1st, 2021 •
1,2, 2, 1, 1, 1, 2,3
1School of Chemical and Environmental Engineering, China University of Mining and Technology, 2State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 3Guangdong Laboratory of Lingnan Modern Agriculture, Shenzhen; Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Præsenteret her er en protokol over Helicoverpa armigera (Hübner) embryomikroinjektion og knockout mutant identifikation skabt af CRISPR / Cas9 genomredigering. Mutante insekter muliggør yderligere forskning i genfunktion og interaktion mellem forskellige gener in vivo.

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Adfærd Udgave 173 Helicoverpa armigera embryomikroinjektion genknockouts CRISPR Cas9
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