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Genetics

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JoVE Journal Genetics
Digital-Droplet PCR om indels mutaties in genetisch gemodificeerde anopheline muggenpopulaties te detecteren
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Digital-Droplet PCR om indels mutaties in genetisch gemodificeerde anopheline muggenpopulaties te detecteren

Article DOI: 10.3791/62607-v • 05:51 min • June 28th, 2021
June 28th, 2021 •
1, 2, 1, 1, 1,2
1Department of Microbiology & Molecular Genetics, University of California, Irvine, 2Department of Molecular Biology & Biochemistry, University of California, Irvine

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Dit protocol biedt de stappen van DNA-extractie tot experimentele opstelling voor digitale druppel PCR (ddPCR), inclusief analyse voor de identificatie en kwantificering van niet-homologe end-joining (NHEJ) gebeurtenissen op doellocaties na gRNA-geïnduceerde Cas9-splitsing en DNA-reparatie. Andere toepassingen van deze methode zijn toepassingen zoals polymorfismedetectie en genbewerkingsvariantverificatie.

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Genetica Nummer 172 polymorfisme DNA-sequentie niet-homologe eindverbinding ddPCR mutagenese DNA-extractie
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