Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Anpassning av gastrointestinala organoider för…
JoVE Journal
Immunology and Infection

Ce contenu est disponible gratuitement.

JoVE Journal Immunology and Infection
Anpassning av gastrointestinala organoider för patogeninfektion och encellssekvensering under biosäkerhetsnivå 3 (BSL-3) tillstånd
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
Journal (Immunology and Infection)
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
Journal (Immunology and Infection)
A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
 
Click here for the English version

Anpassning av gastrointestinala organoider för patogeninfektion och encellssekvensering under biosäkerhetsnivå 3 (BSL-3) tillstånd

Article DOI: 10.3791/62857-v • 07:59 min • September 10th, 2021
September 10th, 2021 •
1,2, 2,3,4
1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, 3Department of Infectious Diseases, Virology, Heidelberg University Hospital, 4Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)

Chapitres

résumé
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Detta protokoll beskriver hur man infekterar mänskliga intestinala organoider från antingen deras apikala eller basolateral sida för att karakterisera värd/patogen interaktioner på encells nivå med hjälp av encelliga RNA sekvensering (scRNAseq) teknik.

Tags

Immunologi och infektion nummer 175 humana tarmorganoider encellig RNA-sekvensering biosäkerhetsnivå 3 organoid infektion enteriska patogener apikal och basolateral infektion
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter