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Biochemistry

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JoVE Journal Biochemistry
Måling af proteinomsætningshastigheder i senescerende og ikke-delende dyrkede celler med metabolisk mærkning og massespektrometri
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Måling af proteinomsætningshastigheder i senescerende og ikke-delende dyrkede celler med metabolisk mærkning og massespektrometri

Article DOI: 10.3791/63835-v • 08:52 min • April 6th, 2022
April 6th, 2022 •
1, 1, 2
1Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, 2Translational Gerontology Branch, National Institute on Aging Intramural Research Program, National Institutes of Health

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Denne protokol beskriver arbejdsgangen for metabolisk mærkning af senescerende og ikke-delende celler med pulserende SILAC, ikke-målrettet massespektrometrianalyse og en strømlinet beregning af proteinhalvingstider.

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Biokemi Udgave 182 cellulær ældning massespektrometri SILAC proteinomsætning proteostase aldring neurodegeneration cellekultur nedbrydning syntese stabil isotopmærkning metabolisk mærkning
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